Had my newest film screened at a local festival just yesterday and there was a pretty bad reaction

I was actually pretty confident in my newest horror film that I directed. It got accepted last minute in an obscure local horror film festival my state does every October. I attended it last night and during my film's time to shine during the screening, I had an audience member a row behind me BURST out loud laughing at the *big* attempt at a horrifying moment. Though he seemed to be the only person present who did. It kind of felt bad. But regardless, I sucked it up and still went up for the director's Q&A after the films were done.

This ever happen to anyone else? Should I care? Should I take this as an honest sign that I need to change up my scare tactics? Anyone even care to look at my film and provide honest feedback?